Aim: To evaluate and compare in vitro the antibacterial efficacy of Bioceramic root canal sealer modified with Piper betel leaf and Morinda citrifolia against Enterococcus faecalis.

Materials and mathods: A Bioceramic root canal sealer was mixed with two herbal extracts (Piper betel leaf, Morinda citrifolia) which was divided into two groups. Their antibacterial efficacy was evaluated against standard strains of Enterococcus faecalis at various time intervals using Agar Diffusion Test. The mean zones of inhibition were measured. The statistical analysis was performed using the SPSS software. Analysis of the data between groups was carried out using paired and unpaired t test.

Results: Both groups demonstrated a reduction in the zone of inhibition over time. However, Group 2 (BIO-C sealer + Morinda citrifolia) consistently exhibited a larger zone of inhibition than Group 1 (BIO-C sealer + Piper betel leaf) at each time interval. With p-values all below 0.01, the differences between the groups across the time points are statistically significant.