ARCHIVES
VOL. 7, ISSUE 3 (2025)
In vitro evaluation of the susceptibility level of Enterococcus faecalis to intracanal medications
Authors
Julio Eduardo Alvarez Carrero, Kevin Villota
Abstract
The main objective of this study was to
evaluate the susceptibility of Enterococcus faecalis to intracanal
medications. To achieve this, a comparative in vitro study was conducted
at the Universidad de Los Hemisferios´ microbiology laboratory, using Enterococcus
faecalis strains in contact with conventional tri-antibiotic pastes,
modified tri-antibiotic pastes, and bi-antibiotic pastes to observe the
presence of inhibition zones, which were subsequently measured with a digital
vernier caliper. The bacteria were cultured in sterile Petri dishes containing
bile esculin agar and incubated at 37.5 °C for 24 hours under aerobic
conditions. After this period, the microorganism was inoculated into a test
tube with physiological serum at a turbidity of 0.5 McFarland. Then, 100 μL of
the solution was extracted and deposited in a Petri dish containing the same
agar, and microorganisms were spread using a Digralsky loop. This procedure was
repeated 20 times. The pastes were prepared for study by removing the coatings
from the tablets of the medications, crushing them in a sterile mortar, and
storing them in sterile, amber-colored containers for each drug for 14 and 28
days. The tri-antibiotic pastes (conventional and modified) were mixed in a
1:1:1 ratio, while the bi-antibiotic paste was mixed in a 1:1 ratio.
Subsequently, all pastes were mixed with glycerin (vehicle) in a 1:1
proportion. Wells corresponding to each paste (100 μL) were opened in the
previously inoculated Petri dishes, then incubated at 37.5 °C for 24 hours.
After incubation, inhibition zones were measured using a digital vernier
caliper. These measurements were recorded in Excel and statistically analyzed
using the ANOVA test and HSD de Tukey. The same procedure was repeated at 14
and 28 days with the previously stored medications. The results showed that the
modified tri-antibiotic paste exhibited inhibition zones greater than 27 mm, while
the conventional tri-antibiotic and bi-antibiotic pastes presented inhibition
zones greater than 21 mm. In conclusion, the study demonstrated that the Enterococcus
faecalis strain is highly sensitive to the studied pastes, and storage time
did not affect the effectiveness of any of the medications.
Download
Pages:68-72
How to cite this article:
Julio Eduardo Alvarez Carrero, Kevin Villota "<i>In vitro</i> evaluation of the susceptibility level of Enterococcus faecalis to intracanal medications". International Journal of Dental Sciences, Vol 7, Issue 3, 2025, Pages 68-72
Download Author Certificate
Please enter the email address corresponding to this article submission to download your certificate.