Background: Separation of endodontic instruments is one of the most frequent procedural complications in root canal therapy. A fractured fragment may obstruct access to the apical third of the canal, hindering effective debridement and disinfection, and thereby jeopardizing treatment success.

Case Report: This paper presents a 2 case reports with separated instruments located at different levels within the middle and apical thirds of mandibular molars. Following radiographic and clinical evaluation, staging of the canal orifice was performed. Retrieval of the separated instruments was achieved using ultrasonics under dental operating microscope. After successful removal, chemomechanical preparation and obturation were completed to the full working length, followed by appropriate post-endodontic restorations.

Results: Both the cases showed favorable clinical and radiographic outcomes. Patient satisfaction was high, with preservation of tooth integrity and functionality.

Conclusion: Successful management of separated instruments depends on accurate case evaluation, proper use of advanced armamentarium, and operator expertise. Ultrasonics under microscope enables efficient and safe retrieval, while preservation of radicular dentin remains essential for long-term prognosis.