Objectives: The primary aim of this investigation was to evaluate the prevalence of third molar (M3) agenesis among the youth demographic in India.

Materials and Methods: A cross-sectional investigation was designed focusing on individuals aged 13 to 29 years born in the 21st century. Participants were selected from patients who required an orthopantomogram (OPG) for various diagnostic reasons.

Results: Out of 250 orthopantomograms analyzed, congenital absence of at least one third molar was observed in 78 individuals (31.2%). The most frequent manifestation of agenesis was the concurrent absence of both maxillary third molars, followed closely by the complete absence of all four third molars. The hierarchy of missing teeth by dental notation was determined to be 18 > 28 > 48 > 38. Notably, the maxilla exhibited a significantly higher rate of agenesis compared to the mandible, and a statistically significant predilection was observed in female participants.

Conclusion: The occurrence of third molar agenesis is rising rapidly in younger population.