Aims and objectives: To evaluate and compare the cytotoxicity of bioceramic pulp capping materials i.e MTA Plus, Biodentine, and Endocem Zr on stem cells derived from human dental pulp and to assess the adhesion of the hDPSCs on these bioceramic pulp capping materials.

Methods and materials: hDPSCs were isolated from extracted maxillary premolars by using the explant method. Material disks were made using a brass mould of diameter 10 mm and thickness 2 mm. Set materials were placed in 24 well polystyrene tissue culture plates and seeded with 20,000 cells/well. After 1,3 and 7 days of incubation, cell viability was measured using MTT assay with microplate reader at 570 nm. SEM was used to visualize the attachment of hDPSCs on the surface.

Statistical analysis used: Kruskal Wallis for intragroup and Friedman’s test for intergroup comparison.

Results: Biodentine showed increased cell viability after 7 days. No statistically significant difference was observed in inter and intragroup comparison. MTA Plus had higher cell attachment when compared to other materials.