Aim: The aim of the study is to evaluate the efficiency of different endodontic irrigants; Irritrol, 0.1%Polyhexanide, and 17%Ethylenediaminetetraacetic acid in removing smear layer using scanning electron microscope through in vitro method.

Materials and Methods: The present in vitro study was carried out on 45 singles‑rooted extracted human mandibular premolar teeth with single canal and complete root formation. Teeth were randomly assigned to three groups with 15 teeth in each group. Group I samples were irrigated with 17% ethylenediaminetetraacetic (EDTA) irrigation, Group II with 0.1% polyhexanide irrigation, and Group III with twin kleen irrigation. Scanning electron microscope evaluation was done for the assessment of smear layer removal in the coronal, middle, and apical thirds. Comparison of the smear layer removal between the three different groups was done by Kruskal–Wallis test, followed by Mann–Whitney U test for comparing individual groups. A “P” value less than 0.05 was considered to be statistically significant.

Results: Statistically significant difference was seen between the two test groups (17% EDTA vs. twin kleen) in smear layer removal at coronal, middle, and apical thirds of the root canal. The most efficient smear layer removal was seen in Group I with 17% EDTA irrigation followed by 0.1% polyhexanide and the least by twin kleen in apical third.